|
KMID : 0620920080400020196
|
|
Experimental & Molecular Medicine
2008 Volume.40 No. 2 p.196 ~ p.207
|
|
|
T-CAM, a fastatin-FIII 9-10 fusion protein, potently enhances anti-angiogenic and anti-tumor activity via αvβ3 and α5β1 integrins
|
|
Nam Ju-Ock
Lee Byung-Heon
Kim In-San
Jung Mi-Yeon
Thapa Narendra
Park Rang-Woon
|
|
|
Abstract
|
|
|
|
We made fusion protein of fastatin and FIII 9-10, termed tetra-cell adhesion molecule (T-CAM) that can interact simultaneously with α vβ 3 and α 5β 1 integrins, both playing important roles in tumor angiogenesis. T-CAM can serve as a cell adhesion substrate mediating adhesion and migration of endothelial cells in α vβ 3 and α 5β 1 integrin-dependent manner. T-CAM showed pronounced anti-angiogenic activities such as inhibition of endothelial cell tube formation, endothelial cell proliferation, and induction of endothelial cell apoptosis. T-CAM also inhibited angiogenesis and tumor growth in mouse xenograft model. The anti-angiogenic and anti-tumoral activity of molecule like fastatin could be improved by fusing it with integrin-recognizing cell adhesion domain from other distinct proteins. The strategy of combining two distinct anti-angiogenic molecules or cell adhesion domains could facilitate designing improved anticancer agent of therapeutic value.
|
|
|
KEYWORD
|
|
|
angiogenesis inhibitors, angiostatic proteins, antineoplastic agents, cell adhesion molecules, integrin α vβ 3, integrin α 5β 1
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
    
|